( 50μL ) Biotinylated Aptamer solution (Tube A ). L ) Biotinylated Aptamer solution (Tube A ). ⢠( 50. ( 50. ( 50μL
Base Pair Biotechnologies is pleased to provide this complete AptaColorTM test kit to easily and quickly demonstrate aptamer specificity in a common lateral flow assay format. Lateral flow assays are based on target immobilization and capillary transfer of detection reagents on a lateral flow strip. The AptaColorTM kit is a simple lateral flow assay designed to specifically detect a protein target with an aptamer developed by Base Pair Biotechnologies. The kit utilizes a biotin labeled aptamer and streptavidin gold conjugate solution that when combined with the target on the lateral flow strip yield a visible pink reaction. The assay demonstrates superb specificity of the aptamer by not binding a non target protein abundant in most biological assays. We would like to thank Diagnostic Consulting Network DCN for the lateral flow strips and conjugate solution.
8058 El Rio St., Houston, TX 77054 • basepairbio.com •
[email protected]
Directions for use: AptaColorTM Kit Materials Include:
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Pulse centrifuge all tubes to accumulate liquids to bottom
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Place strip into 50μ 50μL of the Biotinylated Aptamer solution in 2mL ( Tube A ) , within seconds the liquid will be ab-
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Strip with immobilized Target and Non Target Proteins
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( 5 0 μ L ) Biotinylated Aptamer solution ( Tube A )
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( 5 0 μ L ) StreptavidinStreptavidin-gold conjugate solution ( Tube B )
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2 X ( 50μ 50 μL ) PBS+ solution ( Tube C and D )
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Once absorbed transfer strip to 2mL ( Tube B ) containing 50μ 50μL of the StreptavidinStreptavidin-gold conjugate solution which will also be absorbed into the strip
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Move strip to 2mL ( Tube C ) and rinse for 10 sec.with 50μ 50μL PBS+ solution, repeat with another 50μ 50μL of PBS+ in 2mL ( Tube D )
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Strip should turn pink where the Target Protein has been immobilized ( T ) and not turn pink where the Non Target Protein has been immobilized ( NT ) , dispose of all reagents in a biohazard container.
Base Pair Biotecnologies is not liable for any damages arising from misuse of this kit and contents, use and dispose of all materials as directed.
8058 El Rio St., Houston, TX 77054 • basepairbio.com •
[email protected] 