2.1 mm ID x 100 mm, 0.35 mL/min, 40 C, MS: SQ TIC (+ 300-2000 m/z) @ 0.35/s ... 1) Advanced Materials Technology Inc., 3
Application of Advanced Barry E.
1,2 Boyes ,
® Fused-Core
Stephanie A.
1 Schuster ,
Ron
Particle Technologies to Proteomics
2 Orlando ,
Darryl
2 Johnson ,
and J. Jack
1 Kirkland
1) Advanced Materials Technology Inc., 3521 Silverside Road, Suite 1-K, Quillen Building, Wilmington, DE 19810 2) Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602 Objectives
HILIC Separations with Halo Penta-HILIC
Bonded Phase Ligands
• Evaluate utility of Fused‐Core particle column packing materials for separations of peptides, glycopeptides and glycans.
Penta-HILIC is Useful for Peptide and Glycopeptide Separations with Orthogonal Selectivity Penta-HILIC Resolves Digest, Strongly Retains Glycopeptides
Comparing RP and HILIC Columns for Peptide Separations
250000
Recent developments in HPLC instruments and column packing materials are permitting faster separations, particularly for peptides and protein Larger Pore Fused-Core Particle Permits Fast Separations fragments. The recent popularity of sub‐2 μm diameter particles and new Ultra Fast/High Resolution Separation of apo-Transferrin small diameter superficially porous particles reflect the advantages of Tryptic Digest increased speed and resolution that can be obtained for separations of 0.75 mL/min 5‐60% B in 30 min. peptides, proteolytic fragments, and glycans released from glycoproteins.
50 40
50
30 20
A(17-42)
A(17-40)
A(1-42)
0 2
4
6
8
10
0
12
80 C
80
50
50
40
40
30
30
50
20
20
30
10
10
10
0
0
130 110 90
1.5 mL/min 5‐60% B in 15 min. P
