The Leader in Multiplex Immunoassay Analysis ... analysis with the Bio-Plex System provides .... Bio-Plex Software is th
IL-1β Multiplex System
IL-
TGF-β3
TNF-α
IL-6
M-CSF
GLP-1
TGF-β1
ICAM-1
TRAIL TGF-β1
TGF-β1
TNF-α
CTACK
MIF
TGF-β1
TGF-β1
TGF-β1
MIF
PAI-1
TGF-β1
TNF-α
HGF
TGF-β1
TGF-β1
IL-13
IL-4
TGF-β1 IFN-Υ
TGF-β1 TGF-β1
Bio-Plex Multiplex System ®
TGF-β1
IL-5
TGF-β1
TGF-β1
TGF-β1
The Leader in Multiplex Immunoassay Analysis IL-13
TGF-β1
IL-12p70
TGF-β1
IL-13
TGF-β1
TGF-β1
IL-2
TGF-β1
TGF-β1
TGF-β1
G-CSF
TNF-α
TGF-β1 TGF-β1
TGF-β1
IL-17
TGF-β1
MIP-2
MIP-
IL-10 IFN-γ
IL-12p40
T
The Bio-Plex Multiplex System The Bio-Plex System is a flexible, easy-to-use microplate-based multiplex immunoassay system. It enables you to quantify up to 100 different analytes in a single sample, giving you the power to understand complex relationships among proteins in normal and disease states. Multiplex analysis with the Bio-Plex System provides more information with less sample. The Bio-Plex System comprises all products for a complete workflow — from sample prep reagents and diluents to sophisticated data analysis — for unsurpassed sensitivity, precision, and accuracy.
Bio-Plex 200 Multiplex Reader
With the Bio-Plex System, you can multiplex: n
Immunoassays
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Receptor-ligand assays
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Nucleic acid hybridization assays
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Enzyme assays
Bio-Plex 3D Multiplex Reader
Bio-Plex Pro™ Wash Station
Bio-Plex Pro Assays
BIO-PLEX IMMUNOASSAY WORKFLOW
From Multiplex Assay to Results in as Little as 3 Hours Bio-Plex Assays are designed using microscopic beads, each with a different color code (spectral address) to permit discrimination of individual assays. Beads are dyed with different ratios of two fluorophores and are thus classified into 100 unique bead regions (xMAP technology).
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1. Coupled Beads
2. Samples
A Bio-Plex Assay uses a selection of beads with different spectral 12 12 2 2 addresses, each coupled to antibodies against a different target.
Sample types ranging from serum, urine, milk, and plasma to cell lysates 12 2 with a Bio-Plex Cell Lysis (prepared Kit) can be used with various Bio-Plex Assays.
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Add samples to wells containing coupled beads
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A Multitude of Choices
Bio-Plex Assay Finder Visit bio-rad.com/findmyassay to browse available kits by analyte name, compatible species, and research area.
Bio-Rad offers a continually growing line of assays and associated products for extracellular and intracellular analysis. The assays are relevant to researchers studying everything from cancer to cardiovascular disease and available for use with multiple species and sample types. Go to bio-rad.com/bioplex for the most up-to-date target information.
Find the right Bio-Plex Assay with only a few clicks.
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Cytokines, chemokines, and growth factors
Browse the entire catalog of targets alphabetically
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Filter by species and research area
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Multiplex Assays in a Variety of Panel Configurations for n
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Email your customized search results to facilitate ordering Contact a Bio-Rad representative who can talk to you about your options
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Isotyping
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TGF-b
Acute phase
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The Th17 pathway
MMP and TIMP
Bio-Plex Assay Builder
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Cell signaling
Inflammation
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Design your own custom assays from our selection of hundreds of analytes. Go to bio-rad.com/assaybuilder to learn more.
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Cancer
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Diabetes
Kidney toxicity and kidney damage
Develop Your Own Assay Develop unique bead-based multiplex assays with Bio-Plex Pro COOH Beads. Bio-Plex Pro COOH Beads are carboxy-coated beads ready to be covalently coupled to the protein or nucleic acid of your choice.
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= sVEGFR (5.0 pg/ml) = sHER-2 (1.4 pg/ml)
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= IL-6Ra (3.7 pg/ml)
Bio-Plex Software is the ideal solution to reduce errors, save time, and get up to speed fast.
Reduce errors n
Importing results directly into a database from Bio-Plex Manager data files drastically reduces the need to audit results and eliminates mistakes introduced by copy-andpaste errors
Save time n
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Editing analysis options results in instantaneous updates to all the outputs and reduces the time spent creating graphs and performing analysis Automating calculations based on the most common comparisons used by biological researchers eliminates time spent organizing data Applying a truly integrated solution simplifies and speeds up data analysis
BIO-PLEX SOFTWARE
Bio-Plex Manager™ Software is the ideal tool for reviewing run data and optimizing results from any xMAP instrument. Bio-Plex Data Pro™ Software combines multiple instrument runs to efficiently understand what your multiplex data say about your biological system. Try Bio-Plex Data Pro free for 30 days — Visit bio-rad.com/ad/datapro to download it.
Get up to speed fast n
Truly user-friendly software removes training burden, ensures instrument reliability, and eliminates user error
The Power of Bio-Plex Assays Bio-Plex Multiplex Assays deliver more data in less time with less sample and with fewer manual manipulations. The examples to the right show a comparison between a Bio-Plex Assay and conventional methods. In the top panel, a Bio-Plex Phosphoprotein 5-Plex Assay is compared with 5 western blots. On the bottom panel, a Bio-Plex Human Cytokine 27-Plex Assay is compared with 27 ELISA assays.
Analysis of 5 phosphoproteins in 12 samples. Western Blot 5
5
Number of samples
12
12
Total data points
60
60
Number of gels or 96-well plates Data points per gel or plate Total time required
5
1
12
60
~8 hr + one overnight incubation
Sample volume
2 hr + one overnight incubation 50 µl
125 µl
Dynamic range
Proven Results
Bio-Plex System
Number of phosphoproteins
Limited total protein
0.04–10 µg/well*
* The range of total lysate protein in which the analyte can be detected.
The Bio-Plex System is the most widely cited multiplex assay platform in life science research today. Application areas include inflammation, autoimmune disease, asthma, diabetes/obesity, cancer, neurological disorders, cardiovascular disease, drug discovery, and vaccine development.
Analysis of 27 cytokines in 80 samples. ELISA
Bio-Plex System
27
27
Number of cytokines Number of samples Total data points
Open Platform
2,160
2,160
Number of 96-well plates
27
1
Data points per plate
80
2,160
>60 hr
3 hr
Total time required
Bio-Plex Pro Magnetic Assays configured on MagPlex Beads are compatible with all xMAP life science instruments supplied by any Luminex partner. Choose from a broad selection of preconfigured and custom assay panels.
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Sample volume
Serum or plasma, Serum or plasma, >1 ml* 12.5 µl Cell culture supernatant, Cell culture supernatant, >1 ml* 50 µl
* Based on 50 µl/well of sample.
Bio-Plex Manager Software supports all Luminex 100 and 200 instruments. In addition, it supports both magnetic and nonmagnetic beads and can analyze data from any xMAP platform. Kits are available to run Bio-Plex Manager on Luminex Assay Readers. For more information, contact your local Bio-Rad sales representative.
IL-23
Luminex, MagPlex, and xMAP are trademarks of the Luminex Corporation. The Bio-Plex Suspension Array System includes fluorescently labeled microspheres and instrumentation licensed to Bio-Rad Laboratories, Inc. by the Luminex Corporation. CST Antibodies developed and validated for Bio-Plex Phosphoprotein and Total Target Assays.
IL-1α
TGF-β3
TNF-α
IL-6
M-CSF
IL-17
TGF-β1
ICAM-1
TRAIL TGF-β1
TGF-β1
TNF-α
CTACK
MIF
TGF-β1
TGF-β1
MIF
TGF-β1
PAI-1
TGF-β1
TNF-α
HGF
TGF-β1
TGF-β1
IL-13
IL-4
TGF-β1 IFN-Υ
TGF-β1 TGF-β1
IL-5
TGF-β1
TGF-β1
TGF-β1
TGF-β1
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