late spring 2017 new products & special offers from appleton woods

LATE SPRING 2017 NEW PRODUCTS & SPECIAL OFFERS FROM

APPLETON WOODS

products visit our website at

April 2017• Please quote LSPR’17 on your order • Prices valid until 16/06/2017

www.appletonwoods.co.uk

For the complete range of

TELEPHONE 0121 458 7740

E Q U I P M E N T, C O N S U M A B L E S A N D R E A G E N T S F O R L A B O R A T O R I E S NEW PRODUCTS

AppGreen Blue qPCR Mixes Appleton

Pioneer ® Plus Balances Ohaus

30%

Versatile weighting up to 4 decimal places

OFF

25%

Sensitive qPCR with easy sample visualisation

OFF See page 2

See page 6

PTR-35 Tube Rotator

FiveEasy™ & FiveEasy Plus™ pH/mV Benchtop Meters

Grant Rotation, reciprocation and vibration – all in one!

FREE Platform worth £44

See page 10

Metler Toledo pH/Mv measurement made easy!

25% OFF See page 10

EX TR A SAVINGS

Swiftlock Autoclaves Astell ✔ Benchtop or top loading ✔ Manual or auto-fill ✔ Colour touch screen

Primovert Inverted Microscopes ZEISS ✔ Superior optical quality ✔ Ideal for assessing cells ✔ Easy to use

SAVE

20%

£780

OFF

See page 11

See page 7

BEST SELLERS

Nitrile Gloves, Powder Free Ecoshield™ ✔ For everyday lab use ✔ Category 3 PPE certified

High Speed Vortex Mixer, VX-200 Labnet ✔ Small ✔ Reliable ✔ Efficient

BUY ONE GET ONE

*

FREE

See page 4 See page 4 See page 4

Find us on hosted catalogues

sciencewarehouse

ONLY

£109

PCR and qPCR Reagents Sensitivity, Specificity and Convenience!

• • •

AppGreen Blue qPCR Mixes Appleton For high performance fast, specific and sensitive qPCR using AppGreen (a proprietary intercalating dye which does not interfere with qPCR) AppGreen Blue Separate Easy visualisation and pipetting ROX Mix (2x) using a non-reactive blue dye CODE

Pre-added, optimised levels of MgCl2 and dNTPs for highly reproducible single-gene qPCR

AppGreen Blue Low ROX Mix (2x) CODE

ARP122

REACTIONS

200

REACTIONS

200

ARP142

30% OFF

RRP

£££

100.00

70.00

ARP143

500

240.00

168.00

ARP145

5000

2,200.00

1,540.00

AppGreen Blue High ROX Mix (2x)

RRP

£££

100.00

70.00

ARP132

CODE

REACTIONS

200

RRP

£££

100.00

70.00

ARP123

500

240.00

168.00

ARP133

500

240.00

168.00

ARP125

5000

2,200.00

1,540.00

ARP135

5000

2,200.00

1,540.00

• • •

•

AppScript cDNA Synthesis Kit For reproducible and unbiased cDNA synthesis from 5' and 3' transcripts Synthesize cDNAs up to 9kb with ultra-stable reverse transcriptase High cDNA yields from as little as 4pg total RNA or 0.2pg oligo dT mRNA

•

6.000

Figure 1a & 1b : qPCR amplification curves (1a) and corresponding melt curves (1b) of serial dilutions of mouse cDNA made using the AppScript cDNA synthesis kit.

Amplification Curves

5.000

Fluorescence

Appleton

NE W

Reduced transcript bias for downstream qPCR with pre-optimised oligo dT, random hexamers, enhancers, dNTPs and MgCl2

4.000

A 10 fold serial dilution of mouse total RNA was used for 4 cDNA synthesis reactions.

3.000 2.000

The highest amount of RNA used for cDNA

1.000

synthesis was 5µg and the lowest 5pg. Each cDNA synthesis reaction was used as a

Figure 1a

template for an AppGreen qPCR reaction, amplifying an 80bp fragment of the mouse

0.000 4.00

Short protocol (30mins only) and simple two tube system

8.00

12.00

16.00

20.00

24.00

28.00

32.00

36.00

40.00

44.00

Cycle

GAPDH gene. Reactions were incubated for

0.018

30%

AppScript cDNA Synthesis Kit

30 minutes at 42°C. The amplification curves are four 10 fold serial dilutions of cDNA prepared from total mouse RNA. The equal spacing in Figure 1a demonstrates close to 100% amplification efficiency. The overlapping melt curves demonstrate that the same product was produced from each template dilution. The AppScript cDNA synthesiskit efficiently produces cDNA from a wide range of RNA concentrations.

Figure 1b

0.014 0.012

-dF/dT

OFF

Melt Curves

0.016

0.010 0.008 0.006

CODE

REACTIONS

RRP

£££

0.004

ARP601

25

95.00

66.50

0.002

ARP602

100

342.00

239.40

0.000

ARP603

250

813.00

569.10

1.75

Appleton

1.50

Pre-added, optimised levels of MgCl2 and dNTPs for highly reproducible single-gene or multiplex qPCR

68.00

72.00

76.00

80.00

84.00

88.00

92.00

96.00

Temperature

5.000

Figure 1

Figure 2

4.000

1.25

Intensity (RFU)

For high performance qPCR using probedetection technologies like Taqman, Molecular Beacons and Scorpion probes

64.00

Fluorescence

• •

App Probe qPCR Mixes

60.00

1.00

3.000 2.000 1.000 0.000

0.75

4.00

8.00

12.00 16.00 20.00 24.00 28.00 32.00 36.00 40.00 44.00

Cycle 0.50

Figure 1: Multiplex qPCR of 5 genes using human cDNA and App Probe High ROX Mix (2X). The qPCR mix efficiently generates distinct and reproducible traces for each gene under fast cycling conditions.

Figure 2: qPCR data traces generated using App Probe High ROX Mix (2X). 10-fold serial dilutions of mouse beta-actin cDNA were amplified using Taqman probes labelled with Cy5 dye. Four overlapping replicates demonstrate close to 100% efficiency.

0.25

0.00 5

10

15

20

25

30

35

40

45

30%

Cycle

AppProbe High ROX Mix (2X) CODE

ARP402

REACTIONS

200

AppProbe Low ROX Mix (2X) RRP

£££

90.00

63.00

CODE

ARP302

REACTIONS

200

AppProbe No ROX Mix (2X) RRP

£££

90.00

63.00

CODE

ARP502

REACTIONS

200

OFF

RRP

£££

90.00

63.00

ARP403

500

225.00

157.50

ARP303

500

225.00

157.50

ARP503

500

225.00

157.50

ARP405

5000

2,000.00

1,400.00

ARP305

5000

2,000.00

1,400.00

ARP505

5000

2,000.00

1,400.00

2

Sales & Customer Services 0121 458 7740

Email [email protected]

Please quote LSPR’17

• • • • • • •

AppTaq Polymerase and Ready-mixes Appleton

• • • • • • •

HiFi-App Polymerase Appleton

UP TO

30%

For routine PCR of amplicons up to 6kb

OFF

Pre-optimised buffer system with pre-added dNTPs and MgCl2 Works under standard or fast cycling conditions Clone PCR products into TA vectors Amplifies from complex GC- rich or AT-rich templates

PCR amplification of a 2kb fragment of the HPRT1 gene using AppTaq Polymerase

Suitable sample types: mammalian genomic DNA, GC- or AT-rich DNA

•

AppTaq Polymerase

Competitor N

Available as ready mix for extra convenience or with a red dye for visualising PCR products by agarose gel electrophoresis

0.6pg

12.5pg

0.25ng

5ng

100ng

Human genomic DNA was serially diluted 20 fold from 100ng to 0.6pg

30%

50 fold higher fidelity than AppTaq Polymerase

OFF

Amplify PCR products up to 10kb Superior PCR yields using highly optimised buffer system with pre-added dNTPs and MgCl2 Robust performance under standard or fast cycling conditions (shorter PCR runs) Amplifies from complex GC- rich or AT-rich templates Generates blunt ended PCR products for downstream applications Suitable sample types: complex templates / crude samples / colonies

PCR amplification of a 5kb fragment of the PGK1 gene using HiFi-App Polymerase under fast cycling conditions (