Kanamycin sulfate is an aminoglycoside antibiotic, and is often used as a selective agent to isolate cells that have bee
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ABOUT TOKU-E COMPANY TOKU-E Company is an ISO 9001:2008 certified company with over 25 years of experience and expertise in antimicrobial fermentation, manufacturing, and purification technologies. TOKU-E Company is a primary manufacturer of research antimicrobials for use in numerous in vitro applications including eukaryotic cell culture, microbiology, plant biology, virology, and cancer research. As a global supplier to scientists and notable life science companies, TOKU-E Company is committed to manufacturing antimicrobials of the highest quality and consistency to facilitate reproducible and meaningful research around the world.
PLANT BIOLOGY ANTIMICROBIALS Antimicrobials are often used in plant tissue culture applications to control microbial infection (bacterial, viral, and fungal), select for generically transformed plants, and to eliminate Agrobacterium after successful transformation. Choosing the correct antimicrobials to use in plant cell culture can be challenge due to their toxicity to various plant tissues. The antimicrobials listed below have low toxicity to plant tissue and are recommended for use in plant tissue culture applications. For protocols, applications, and a complete list of products, visit www.TOKU-E.com. *Offer valid until 8/31/17
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Bialaphos sodium
Timentin
Bialaphos sodium has successfully been used in major cereal species transformation including (wheat, rice, maize, barley, sorghum, oat and rye). The ‘bar’ gene was in these cases successfully incorporated in the plant genome, together with the gene of interest.
Timentin is used in Agrobacterium mediated transformations to control Agrobacterium growth. Carbenicillin and cefotaxime can be used in the same applications, however, a study by Nauerby et. al. (1997) found timentin to be less toxic.
• Bialaphos sodium (B013)
• Timentin (T020)
Cefotaxime sodium
Carbenicillin
Cefotaxime is often used in Agrobacterium tumefaciens mediated transformations to manipulate bacterial growth. It is also sometimes used in combination with Vancomycin as it has a synergistic effect.
Carbenicillin disodium is a beta-lactam antibiotic that is often used to select for cells that have been transformed with a plasmid containing the ampR gene which confers resistance to ampicillin and carbenicillin.
• Cefotaxime sodium, USP (C011) • Cefotaxime sodium, UltraPure (C106)
Hygromycin B Hygromycin B is an aminoglycoside antibiotic and is commonly used to select for transfected cells containing the hyg or hph resistance genes.
• Carbenicillin disodium, USP (C126) • Carbenicillin disodium, UltraPure (C107)
Kanamycin
• Hygromycin B (H007) • Hygromycin B, 50 mg/ml solution (H011)
Kanamycin sulfate is an aminoglycoside antibiotic, and is often used as a selective agent to isolate cells that have been successfully transformed with plasmids containing the kanamycin resistance gene, nptII.
Gentamicin sulfate
• Kanamycin sulfate, USP (K008) • Kanamycin acid sulfate (K004)
Gentamicin sulfate can be added to cell culture media to prevent contamination by Gram-positive and/or Gram-negative bacteria for maintaining continuous cell lines or when propagating primary cell lines. • Gentamicin sulfate, USP (G006) • Gentamicin sulfate, EP (G007) • Gentamicin sulfate, 50 mg/ml solution (G046)
Nystatin Nystatin is a fungicidal and fungistatic polyene antimycotic compound and can be used to treat and control fungal contamination. • Nystatin (N010)
Streptomycin sulfate
Rifampicin
Streptomycin sulfate is an aminoglycoside antibiotic and inhibits protein synthesis in Gram-positive and Gram-negative bacteria.
Rifamipicin is an antibiotic that inhibits protein synthesis and can be used to control contamination by Mycobacteria, Gram-positive, and Gram-negative bacteria.
• Streptomycin sulfate, USP (S008) • Streptomycin sulfate, EP (S040)
• Rifampicin (R003) DISTRIBUTED BY:
Vancomycin HCI Vancomycin HCI is a glycopeptide antibiotic and inhibits peptidoglycan synthesis in Gram-positive bacteria. • Vancomycin HCI (V001)
Tel: EU +32 (0)92 61 69 70 | Tel: US (1-888-71-TOKUE) | Tel: SG +65 6816-2640 | Email:
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