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Aug 2, 2007 - Keywords: Apis mellifera L., Reproductive Physiology, Miticides, ..... 50% loss of haemolymph protein content and total haemolymph ..... two-branched spermathecal gland plays a role in the delivery of spermatozoa into the.
The Effects of Miticides on the Reproductive Physiology of Honey Bee (Apis mellifera L.) Queens and Drones

Lisa Marie Burley Thesis submitted to the faculty of Virginia Polytechnic Institute and State University in partial fulfillment of the requirements for the degree of: Master of Science in Life Sciences

Advisory Committee: Dr. Richard D. Fell, Chair Dr. Carlyle C. Brewster Dr. Donald E. Mullins Dr. Richard G. Saacke August 2, 2007 Blacksburg, Virginia Tech Keywords: Apis mellifera L., Reproductive Physiology, Miticides, Spermatozoa viability, Numbers of Spermatozoa, Honey Bee, Queen, Drone Copyright 2007, Lisa Marie Burley

The Effects of Miticides on the Reproductive Physiology of Honey Bee (Apis mellifera L.) Queens and Drones Lisa Marie Burley

Abstract The effects of miticides on the reproductive physiology of queens and drones were examined. The first study examined the effects of Apistan® (fluvalinate), Check Mite+ (coumaphos), and Apilife VAR® (74% thymol) on sperm production and viability in drones. Drones from colonies treated with each miticide were collected at sexual maturity. Sperm production was determined by counting the number of sperm in the seminal vesicles. Sperm for viability assays was analyzed by dual fluorescent staining. Apilife VAR® and coumaphos significantly lowered (P